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Intriguingly, this effect required continuous exposure to APAP, as animals fed APAP overnight (to simulate a brief/singular dose) showed a mild reduction in survival, but the reduction in survival was not as significant as those who experienced continuous exposure (Supplemental Fig

This in turn, was accompanied by activation of PARP, suggesting the activation of DNA repair mechanism and apoptotic signals in ASA treated cells

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So GSH is separate in effect and potential for direct issues that other thiols like ALA and NAC as well as the thiol stabilizer Silibinin