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At the end of the experiment, the area with new hair covering in the treated skin was measured, and the skin of each animal was harvested for subsequent studies

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Pickart estimated lethal dose at approximately 22,500 mg for humans (300-fold above effective doses), with animal LD50 studies in mice showing 8 mg per 25g mouse (320 mg/kg, translating to ~22,400 mg for 154 lb human)