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Cells were then washed with PBS, fixed with cold 1:1 methanol and acetone for 3 min and permeabilized with 0.2% Triton X-100 for 5 min

Mol Biol Rep 37:8792 Blankenberg S, Rupprecht HJ, Bickel C, Torzewski M, Hafner G, Tiret L, Smieja M, Cambien F, Meyer J, Lackner KJ (2003) Glutathione peroxidase 1 activity and cardiovascular events in patients with coronary artery disease