H&E staining revealed only minor nuclear condensation in the ART group, whereas the DOX and MND groups showed increased areas of nuclear condensation, with MND also displaying nuclear dissolution, indicative of apoptosis and necrosis
Isotope-labelled PGRMC1 proteins for NMR analyses were prepared by growing cells (BL21 (DE3)) in minimal M9 media in H 2 O or 99.9% 2 H 2 O, including ampicillin, metals, vitamins, 15 N-ammonium chloride and 13 C or 12 C glucose as sources of nitrogen and carbon, respectively
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