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Rats were housed in a humidity- (50 5%) and temperature-controlled (23 2) room, under a 12/12 h light (200 lux)/dark cycle, with rodent standard chow and water available ad libitum

In the case of ATP, access of extracellular ATP to the cytosol requires cell permeabilization as no cellular transporters are available to equilibrate ATP in the extracellular and intracellular space