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Furthermore, 18.5 l of 1 M potassium hydroxide, 30 l of 150 mM monopotassium phosphate (KH2PO4)/150 mM potassium chloride (KCl) solution (Ph 6.0) and 0.5% acetonitrile were added

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It is capable of binding protein in both native and denaturing conditions.The use of denaturing condition of becomes important when proteins are found in inclusion bodies and must be denatured so they can be solubilized

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