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Anti-ferroptotic compounds that reduce oxidative stress include liproxstatin 1 (Lip-1) (inhibitor of ROS and lipid peroxidation), chalcones 14a-c (inhibitor of A and lipid peroxidation), Selenomethionine (Se-Met) (inhibitor of lipid peroxidation), CMC121 (fatty acid synthase inhibitor), N -acetylcysteine (NAC) (free radical scavenger), Vitamin E (Vit E) and PD146176 (15-LOX-1 inhibitor)

Chander et al

Blood was drawn from the vena cava and transferred into a 1.5 mL Eppendorf tube (Eppendorf) for serum analysis or into EDTA-coated tubes (Micro tube 1.3 mL, Sarstedt)

Immunoblot analysis of oxidized and glutathionylated proteins To measure the level of nuclear protein oxidation 10 g of nuclear lysates were derivatized, and the western blotting was performed according to the recommendation of the manufacturer