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PEG is currently the most popular and practical material to passivate NP surfaces by shielding surface charges, imparting longer circulation time, and more cellular uptake of PEGylated NPs (Joralemon et al., 2010)

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In silico Analysis Pathogenicity of the identified variant was evaluated with different online mutation prediction tools such as MutationTaster (mutationtaster.org), DNN ( FATHMM (fathmm.biocompute.org.uk), LRT (genetics.wustl.edu/jflab/lrt_query.html), VarSome ( CADDCombined Annotation Dependent Depletion ( and MutationAssessor (mutationassessor.org)