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p53 beta-lactamase reporter gene cellular functional assay HCT116 cells were stably transfected with a p53 responsive -lactamase reporter and expanded in McCoys 5 A Medium with 10% fetal bovine serum (FBS), Blasticidin, and Penicillin/Streptomycin and then transferred to 1.5 ml freezing vials and stored under liquid nitrogen in growth media containing 5% DMSO

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(2) Risks to subjects are reasonable in relation to anticipated benefits, if any, to subjects, and the importance of the knowledge that may be expected to result