glutathione sepharose beads protocol Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP lytic enzymes Pierce™ Glutathione Agarose 10 mL

The Angiogenesis Analyzer tool of ImageJ software19 provided the statistical analysis for each experimental condition tested

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Solution selection and preparation The solution used to suspend the crude NPC suspension and to dilute the OptiPrep may be a routine buffered saline such as PBS [4,5], which may be supplemented with 1% BSA [6] or a balanced salt solution such as Hanks Balanced Salt Solution (HBSS) [7-11] or an NPC customized medium such as Geys balanced salt solution [12-15] and this may be prepared as described in the box

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