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ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, Minneapolis, MN, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) before procedure, after ligation and therapy application, and before sacrifice

To achieve stable, long-term exposure baselines in your cell culture models, space your testing intervals systematically rather than administering a single massive concentration all at once

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