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Description

The wild-type amplicon is 250 bp, whereas the amplicon size is 1039 bp upon 3HA knock-in

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Mannoprotein release was indirectly determined by quantifying the concentration of mannose in the supernatant of the suspensions, after hydrolysis with sulfuric acid (final concentration of 1.5 M) at 100C for 90 min and subsequent neutralization with NaOH (2 M)