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was higher than that of the control group, and the number of Escherichia and Staphylococcus counts were lower than the control group ( Lactobacillus and Bifidobacterium in the intestinal tract and a decrease in the number of Staphylococcus in the intestinal tract compared with that of the control group before the treatment ( Enterobacteriaceae and Enterococcus were lower than before treatment, and the numbers of Lactobacillus and Bifidobacterium microbiome were higher than before treatment ( 4 Mechanisms of dysbiosis on GERD Ecological dysbiosis is an abnormal state of the microbial ecosystem in the host ( Figure 1 )

J Bacteriol 87:468472 Brown J, Benedict K, Park BJ et al (2013) Coccidioidomycosis: epidemiology

doi: 10.1007/s12603-015-0636-3

Real-time reverse transcription-polymerase chain reaction (RT-qPCR) Dissected tissues were subjected to total RNA extraction using TRI reagent (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturers instructions