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Negative ion tandem mass spectrometry for the detection of glutathione conjugates

These individuals had elevated plasma ratios of Cys to sulfate due to lower sulfate and higher Cys concentrations, excreted a smaller percentage of a dose of acetaminophen as the sulfate versus the glucuronide conjugate, or had a lower sulfate concentration of synovial fluid, all of which are consistent with impaired Cys oxidation at the CDO level

At the subcellular level, these proteins have a peroxisomal localization, which is consistent with the presence of C-terminal SK[I/M] targeting motif (Dixon et al., 2009)

Alpha-fetoprotein: past, present, and future