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Whole cell lysate protein extraction EEC cell pellets were thawed on ice and resuspended in 2 mL RIPA buffer prepared as described above

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SREBP-2 is a metabolic gene that activates cholesterol and a member of the liver cholesterol metabolism transcription factor, whereas SREBP-1c is largely expressed in liver tissue and is in charge of controlling the expression of genes associated to fatty acid production (Hampton 2002)

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