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Proteins were precipitated by adding four volumes of ice-cold acetone and subsequent centrifugation (21.000 g , 20 min, 4 C)

A recent study also discovered that Mn accumulated in dopamine neurons and increased excitability, reduced amplitude, and half-width of action potentials (Lin et al., 2020)

ALC steps in as a beacon of hope, providing essential nutrients, which is vital for energy production in neuronal cells