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Immunofluorescence analysis The 8-well plates were subdivided into groups to incubate the cells according to the experimental requirements and were dosed with drugs in time

Put a new needle on the pen (always use a new needle and dont share needles)

CysteineGSH axis in fibrosis control GSH, a key intracellular antioxidant, is synthesized from glutamate, cysteine, and glycine, with glutamatecysteine ligase (GCL) catalyzing the rate-limiting step[119]

References Singh R, Tiwari MK, Gangopadhyay D, Mishra PC, Mishra H, Srivastava A, Singh RK (2018) Detection and monitoring of in vitro formation of salicylic acid from aspirin using fluorescence spectroscopic technique and DFT calculations