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The medium was removed following treatment, and the cells were incubated for 30 min at 37C in a buffer containing 10 M of H2-DCFDA

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Linear Discriminant Analysis (LDA) and Partial Least Squares Regression (PLSR) provided qualitative and quantitative evaluations over a wide concentration range (0.150 mol L 1 ), yielding detection limits of 0.51.3 mol L 1 for individual biothiols