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The insertion technique should be executed with gentleness to avoid harming the abdominal organs

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Following transfection, the cells were lysed with reporter lysis buffer (Promega), and luciferase activity was measured with the Dual-Luciferase Reporter Assay System (Promega, E1910) according to the manufacturer's protocol and quantified using a GloMax 20/20 Luminometer (Promega, USA)