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It was found that the treatment of leukocytes with bleomycin induced DNA damage, and the addition of selenomethionine significantly reduced bleomycin-induced DNA breaks and improved the repair of DNA damage (162)

An increase in dietary arachidonic acid (AA) enhances the atherogenic effect associated with this genotype, whereas an elevated intake of omega-3 fatty acidsspecifically eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) mitigates this effect [112]

For this reason, early studies using this design should not enrol people who have advanced HIV (for example with CD4 counts less than 100 cells/mm 3 )

Since then, several multi-metabolite methods have been published, covering these compounds, with some examples of recently applied methods listed in Table 3