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Lysates were centrifuged at 7,000 g for 10 min by MX-100 (Tomy Seiko, Tokyo, Japan), and the supernatant was applied to GST Spin Trap column (GE Healthcare Life Sciences) and kept it for 10 min at room temperature

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Int J Mol Sci 17(9):1401 Marceau F, Bawolak M-T, Lodge R, Bouthillier J, Gagn-Henley A, Ren C, Morissette G (2012) Cation trapping by cellular acidic compartments: beyond the concept of lysosomotropic drugs