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10.25011/cim.v37i3.21379 104 XuC.SunL.RenF.HuangP.TianZ.CuiJ.et al (2020)

References Brcic L, Brcic I, Staresinic M, Novinscak T, Sikiric P, Seiwerth S

(3)Fmoc-Gly-Leu-Val-WangResin (3) Preparation of Fmoc-Gly-Leu-Val-WangResin (2)Fmoc-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Gly-OH0.7gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Gly-Leu-Val-WangResin In the Fmoc-Leu-Val-WangResin of step (2), add decapping reagent (DMF solution of 20% piperidine), react at room temperature for 5 minutes, drain, add decapping reagent at room temperature and react for 20 minutes, wash with isopropanol 3 times, washed 3 times with DMF, washed 2 times with re-distilled DMF, drained, added Fmoc-Gly-OH0.7g, DIEA0.4ml, TBTU0.56g, HOBT0.2g, DMF10ml, reacted at room temperature for 1.5 hours, pumped with a vacuum pump Dry, wash 3 times with isopropanol, wash 3 times with DMF, wash 2 times with DMF that has been distilled, and drain to obtain Fmoc-Gly-Leu-Val-WangResin

For a 0.5mg dose: 0.5 divided by 3.33 equals 0.15mL, which is 15 units