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The fluorescence intensity of the blank control and the experimental group was measured under the appropriate exposure time and gain conditions, and the same detection system and measurement parameters were used in the measurement process to ensure the reliability and accuracy of the fluorescence signal

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In brief, for cells, samples were collected in the same way as described in the Cellular metabolomics analysis section, and 500 l of Solution I was added to each sample, which was subsequently homogenized by ultrasonication