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The term purity is most accurate when applied to raw peptide materials, isolated compounds, or lyophilized peptide powders where a single active compound is measured against potential impurities

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The quantitative real-time PCR (RTqPCR) Cells and tissues had their total RNA extracted using TRIzol (Invitrogen, USA), and RT-qPCR was conducted with a reverse transcription kit (TOYOBO, OSAKA, JAPAN) to synthesize cDNA from RNA