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After the preparation of the dot blot membranes and the 1 h blocking in tris-buffered saline (TBS) with 0.05% Tween 20 (TBST) and 5% non-fat milk, dot blot membranes were incubated at room temperature for 1 h with the primary antibody and for another hour with the secondary antibodies

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The research was inspired by a stroke survivor who claimed he simply forgot his two-pack-a-day addiction no cravings, no nicotine patches, not even a conscious desire to quit