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344 Iron overload may trigger an inflammatory response through activating the NF-B pathway and inducing the secretion of inflammatory factors such as IL-1, TNF-, and IL-6

doi: 10.1016/j.semcancer.2013.05.006 136 ChenEIHewelJKruegerJSTirabyCWeberMRKralliAet al

Gene knockout by CRISPR/Cas9-sgRNAs The sgRNA expression plasmids targeting human SLC13A3 (sgRNA: GATGGTGACAATAGCCACGC) or mouse Slc13a3 ( sgRNA: GGAAGGAGGATGAATATCGT) were constructed by ligating the corresponding annealed guide oligonucleotides to pLenti-U6-sgRNA-EFFS-spCas9-P2A-Puro plasmid co-expressing the Cas9