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Nonmigrated cells were removed with cotton swabs, and migrated cells were fixed with ice-cold methanol and stained with 4,6-diamidino-2-phenylindole (DAPI)

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12 ] In humans, melatonin production often declines with age, which may contribute to sleep disturbances and disruptions in normal biological timing

Intriguingly, appropriate linkers differed among the dimeric macrocycles: (1) aML5 yielded the highest activity when dimerized by PEG3 (aML5-PEG3) and was nearly inactive when dimerized by the other two linkers (Fig