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Caliceti P, Schiavon O, Morpurgo M et al (1995) Physico-chemical and biological properties of monofunctional hydroxy terminating poly(N-vinylpyrrolidone) conjugated superoxide dismutase

Briefly, mice were anesthetized with isoflurane (5% induction, 1.52% maintenance) (CAS 26675-46-7, Laboratorios Karizoo, Spain)

Rats treated solely with rotenone exhibited significantly reduced mitochondrial respiratory function, including basal respiration, ATP production, and maximal respiration

They were then washed with PBS on the rotating bed for 5 min and permeabilized using 0.1% Triton X-100 in PBS for 10 min, followed by another 5 min PBS wash on the rotating bed