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Following incubation with primary antibodies, membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling, 5366S)

The first mechanism includes the accumulation of flavonoids, carotenoids, and vitamins such as ascorbic acid (vitamin C) and -tocopherol (vitamin E), while the latter system includes the induction of enzymes such as peroxidases, superoxide dismutase, glutathione reductase, and others

5) This product is produced in manufacturing facilities such as peanuts, soybeans, peaches, milk, tomatoes, wheat, pork

Shaking introduces air bubbles and mechanical stress that can degrade the compound