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Step 1: concentration Concentration is total peptide mass divided by diluent volume: 5 mg vial 3 mL = 1.667 mg/mL = 1,667 mcg/mL 10 mg vial 3 mL = 3.333 mg/mL = 3,333 mcg/mL Step 2: volume for a target amount Volume equals target dose divided by concentration

Add 1 mL to 2 mL of bacteriostatic water to the lyophilized BPC-157 vial using a sterile syringe, swirling gently to dissolve without shaking

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