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PCR was performed for 50 cycles of 10 min at 95C, 15 s at 95C, and 60 s at 60C using 7,500 Fast Real-Time PCR (Applied Biosystems, United States)

In a nasal colonisation model, production of lipocalin, an acute-phase inhibitor of siderophore-mediated iron uptake, was upregulated but did not affect the ability of H