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The immunoprecipitated proteins and cell lysates were electrophoresed on 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and then transferred to nitrocellulose blotting membranes (GE Healthcare)

7 Diagnostic potential of glycolysis in ischemic stroke Glycolytic metabolic processes, particularly enzymes like HK2, PFKFB3, PKM2, PDH, LHD, and metabolites such as lactate, have emerged as potential predictors of stroke risk, development, and prognosis (39, 59, 196199)

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It is important to understand the causes and risk factors for L-Carnitine deficiency to ensure adequate intake and maintain optimal health