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Peroxiredoxin (Prx) 3, a cysteine-dependent peroxidase enzyme, is also present in the mitochondria, whereas Prx5 can also be found in the cytosol, in peroxisomes, and in the nucleus [28]

Leung LC, Wang GX, Madelaine R, Skariah G, Kawakami K, Deisseroth K, Mourrain P (2019) Neural signatures of sleep in zebrafish

The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation