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Several approaches have been explored to enhance maturation of HLCs, including the formation of tissue-like 3D spheroids or organoids, use of extracellular matrix (ECM)-based hydrogels or scaffolds, coculture with endothelial cells or other hepatic cells, the addition of small molecules uncovered by screening, and overexpression of certain transcription factors, all of which are reviewed elsewhere (46)

While no specific harm has been documented, the absence of research means risks cannot be adequately assessed