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Mitochondria produce a large amount of free ROS in the normal metabolism of electron transport chain and cell energy supply, and endogenous ROS can also be released by microsomes, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, xanthine oxidase, peroxidase, etc

Whole blood was layered on to ficoll histopaque in a 1:1 ratio and centrifuged at 25C for 30 minutes at 1800 RPM with the slow brake to prevent disturbance of the gradient

The patient was definitively diagnosed with IAS