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Figure 1 The biological effects of testosterone are mediated both by direct binding and activation of the androgen receptor, as well as indirectly through its metabolic conversion to biologically active derivatives, including 5-dihydrotestosterone (DHT) via 5-reductase and oestradiol via aromatization, which subsequently act through androgen and oestrogen receptors (ER/ER), respectively

The mobile phase system consisted of two phases, phase A (water) and phase B (water/acetonitrile, 1:9, v/v), and 10 mM of ammonium acetate was added to both of the phases