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Centrifuge cell suspension at 100-200 x g for 5-10 minutes

In the absence of intracellular nucleophiles such as GSH, this reactive metabolite ( N -acetylbenzoiminoquinone) not only reacts with nucleophilic groups of cellular proteins resulting in direct hepatocellular damage, but also participates in redox cycling, thereby generating reactive O 2 species ( ROS ) and consequent oxidative stress that greatly enhance APAP-induced hepatotoxicity

Cryo-EM and artificial intelligence visualize endogenous protein community members

Both AQ and DEAQ are prone to enzymatic oxidation leading to the formation of AQ-QI and DEAQ-QI (Lobach and Uetrecht, 2014