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It is rare indeed to find a study that blinds the administrator of an orthopaedic intervention, as injections and oral medications are not the most prominent tools in our predominantly surgical armamentarium

To address this gap, researchers developed a systematic approach for identifying potential downstream targets based on three rational criteria: (1) expression in brain regions enriched for NRN1, (2) functional relevance to neural excitability through promotion of outward potassium currents, and (3) established connections to neurite growth and development

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Antibodies The following primary antibodies were used: anti-GSTP1 (Rabbit, Proteintech, 15902-1-AP), anti-GAPDH (Mouse, Proteintech, 60004-1-Ig), anti-DYKDDDDK (FLAG) Tag (Rat, Biolegend, clone L5, 637301), anti-Brn2 (Rabbit, Proteintech, 14596-1-AP)