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Then, the bound oligonucleotides were eluted by incubating the binding complex three times with 200 L elution buffer (40 mM Tris-HCl pH 8, 10 mM EDTA, 3.5 M urea, 0.02% Tween 20) at 90 C for 10 minutes

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However, culture-independent barcoding approaches (e.g., PCR-DGGE) revealed the presence of Acidobacteria in AMZ [21], which can comprise up to 84.4% of the total bacterial abundance in AMZ [28]

In particular, the function of the upregulated PH3 (Solyc10g084380.1.1) in PhAN4 HRC is rather to be associated with anthocyanin biosynthesis boosting than with acidification of pH, as tomato lacks PH1 and PH5 (i.e., the genes encoding the vacuolar P-ATPases which cooperate with PH3 and PH4 in determining flower color by hyperacidification of petal cell vacuoles in Petunia )