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The QUIN branch of the KYN shunt is also considered the energy metabolism one, as it leads to the formation of NAD + and branches further, upstream of the QUIN formation, into a different pathway generating acetyl-CoA (35)

In our study of rats liver GST purification, the GSH agarose matrix of affinity chromatographic column retained about 50-60% of the GST activity from liver crude extract and about 40-50% was not fixed to the column matrix this may be attributed to that not all GST isozymes can bind to the affinity column matrix

For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively