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This includes urease and protease, which are critical for nitrogen mineralization and release of ammonium and phosphatases (both acid and alkaline), which catalyze the mineralization of organic phosphorus into plant-available phosphate and -glucosidase and cellulase, which drive carbon cycling by breaking down cellulose and other carbohydrates to facilitate soil organic matter decomposition 44

Assembly of the three PCR products two from both flanking regions of the target gene, one from the antibiotic resistance gene was accomplished using overlap extension PCR with primers hybridizing at both extreme ends, and the resulting DNA fragment was purified by agarose gel electrophoresis

Initial analyses of cancer cell lines found a close correlation between glycine consumption from the biosynthetic pathway and cell proliferation rates (22)