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Following 3 hours of feeding the CR mice at the start of the light cycle, all dietary groups underwent a 3 hr fast, followed by cervical dislocation and tissues for molecular analysis were flash-frozen in liquid nitrogen or fixed and prepared as described in the methods below

The development of technologies such as CRISPRCas9, which is based on targeted DNA editing, has facilitated rapid progress in gene therapy, and these technologies have also shown therapeutic effects in mice with fragile X syndrome