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NET-containing guanylated peptides can be internalized by FLSs through the TLR9 pathway, elevating the inflammatory phenotype of FLSs and upregulating the expression of major histocompatibility complex class II molecules, which subsequently produce autoantibodies by presentation to Ag-specific T-cells (168)

More serious concerns require careful monitoring but affect only a small percentage of patients

Samples were incubated for 1 h under microaerobic conditions with shaking