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To visualize complex IV activity, the gel was washed with 50 mM sodium phosphate buffer (pH 7.2) and submerged in 10 ml of staining solution B (0.075% diaminobenzidine, 50 mM sodium phosphate buffer, pH 7.2), and then 50 l of 5 mM cytochrome c (in 100 mM Tris-HCl, pH 7.5) was added, and the gel was stained overnight by gently shaking in the dark

While many peptides act as receptor agonists or antagonists, GHK-Cu operates through copper-binding chemistry, which enables it to participate in trace-metal transport and copper-dependent enzymatic systems

Strategy of targeting ligand functionalization significantly relies on the receptor expression, and stimuli-triggered BBB disruption has limited resolution

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