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IsoTOP-ABPP Mass Spectrometry Analysis Peptides from all chemoproteomic experiments were pressure-loaded onto a 250 m inner diameter fused silica capillary tubing packed with 4 cm of Aqua C18 reverse-phase resin (Phenomenex, 04A-4299), which was previously equilibrated on an Agilent 600 series high-performance liquid chromatograph using the gradient from 100% buffer A to 100% buffer B over 10 min, followed by a 5 min wash with 100% buffer B and a 5 min wash with 100% buffer A

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Nevertheless, recent investigations by McMullen et al