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If there is a lack of cobalamin in the body, methylmalonyl CoA accumulates, which presumably leads to the neurological manifestations of B12 deficiency.[4][7][9][12] The replenishment with parenteral cyanocobalamin causes a rapid and complete improvement of megaloblastic anemia and gastrointestinal symptoms caused by vitamin B12 deficiency

That indicated poor red blood cell production, and those that were produced were malformed and had short life spans

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To mitigate selection bias, animals are randomly assigned to control and treatment groups