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Inhibition of MTHFD1L or MTHFD2 reduces formate production, whereas ALDH1L2 loss redirects 10-formyl-THF toward formate

SLC39A1 lko mice displayed reduced tumor number in DEN-CCL4 model compared with SLC39A1 flox/flox mice, evidenced by reduced liver damage, hepatocyte proliferation (Ki-67), inflammation (TGF-), and fibrosis (Masson), accompanied by increased ROS accumulation and decreased LC3 and Lamp1

Moving forward, establishing consensus protocols for sample processing, assay selection, data normalization, and reporting standards is essential for validating mtDNA-based biomarkers