glutathione-diabetes typ 1 Improved glycemic control either alone, or combined with antioxidant supplementation, fails to restore blood glutathione or markers of oxidative stress in adolescents with poorly controlled type diabetes Learn about the role of

To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig

10.1042/CBI20110566 Cell Biol

246 Beyond Griess mechanism, Zhang et al

Compounding this enzymatic failure, clinical evidence has demonstrated a significant systemic reduction in the lipophilic antioxidant pool itself