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Glutathione S-transferase (GST) activity was assessed by adding 10 L of the tissue supernatant to a mixture of 10 L of GSH (5 mM) and 10 L of 2,4-dinitrobenzene (CDNB) (1 mM) in 100 L of phosphate buffer, recording at 340 nm for 5 min, and the activity was expressed as moles of CDNB conjugated/min/mg of protein (Al Kury et al., 2019)

The expression of mitochondrial methylenetetrahydrofolate dehydrogenase 2 (MTHFD2), which is necessary for purine synthesis, depends on ATF4

No grain

Weeknight Grilled Pork Tenderloin Ingredients: Pork Tenderloin Chili Powder Paprika Salt & Pepper (to taste) Instructions: Create a dry rub of equal parts paprika and chili powder, along with salt and pepper to taste