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Acta Med Okayama (2009) 63:917

After TMB substrate solution is added, only those wells that contain Mouse LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

Consistently, apigenin and luteolin treatment in MPTP mice was shown to preserve TH levels and significantly reduce striatal TNF-, thereby dampening neuroinflammation in vivo (27)